- Since PCR is carried out at elevated temperature, the DNA polymerase enzyme that is used in the incubation mixture must be heat resistant so that it can be active.
- If it were not heat resistant, the high temperatures would inactivate the enzyme so that it could no longer catalyse its specific reaction. (The DNA polymerase enzyme that is used comes from a bacterial species that lives in hot springs.)
Q6. Explain why the DNA polymerase enzyme used is heat resistant